Cornulin Polyclonal Antibody, Cy3 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-5833R-Cy3 |
| Product Name | Cornulin Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human HSP58/Cornulin |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 49860 |
| Subcellular location | Cytoplasm |
| Synonyms | HSP 58; HSP-58; PDRC1; 53 kDa putative calcium binding protein; 53 kDa squamous epithelial induced stress protein; 58 kDa heat shock protein; C1orf10; Chromosome 1 open reading frame 10; CRNN; DRC1; SEP53; Squamous epithelial heat shock protein 53; Tumor related protein; CRNN_HUMAN; HSP58/Cornulin. |
| Background | This gene encodes a member of the "fused gene" family of proteins, which contain N terminus EF hand domains and multiple tandem peptide repeats. The encoded protein contains two EF hand Ca2+ binding domains in its N terminus and two glutamine and threonine rich 60 amino acid repeats in its C terminus. This gene, also known as squamous epithelial heat shock protein 53, may play a role in the mucosal/epithelial immune response and epidermal differentiation. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Cornulin Polyclonal Antibody, Cy3 Conjugated (bs-5833R-Cy3) validated for, and at what dilution?
bs-5833R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-5833R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Cornulin Polyclonal Antibody, Cy3 Conjugated (bs-5833R-Cy3)?
KLH conjugated synthetic peptide derived from human HSP58/Cornulin
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-5833R-Cy3)?
Cy3 on bs-5833R-Cy3: excitation / emission 512,550nm/570,615nm.