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HEPACAM Polyclonal Antibody, FITC Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human

Predicted Reactivity

  • Mouse
  • Rat
  • Dog
  • Cow
  • Pig
  • Horse
  • Rabbit
Overview
Catalog # bs-5840R-FITC-100uL
Product Name HEPACAM Polyclonal Antibody, FITC Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human
Predicted Reactivity Mouse, Rat, Dog, Cow, Pig, Horse, Rabbit
Specifications
Conjugation FITC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human HEPACAM
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 220296
Subcellular location Cytoplasm, Cell membrane, Extracellular matrix
Synonyms GlialCAM; HECAM_HUMAN; HEPACAM; Hepatocyte cell adhesion molecule; Protein hepaCAM.
Background Involved in regulating cell motility and cell-matrix interactions. May inhibit cell growth through suppression of cell proliferation.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is HEPACAM Polyclonal Antibody, FITC Conjugated (bs-5840R-FITC) validated for, and at what dilution?

bs-5840R-FITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does HEPACAM Polyclonal Antibody, FITC Conjugated (bs-5840R-FITC) react with?

Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Pig, Horse, Rabbit.

How should bs-5840R-FITC be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise HEPACAM Polyclonal Antibody, FITC Conjugated (bs-5840R-FITC)?

KLH conjugated synthetic peptide derived from human HEPACAM

What are the excitation and emission wavelengths of the FITC conjugate (bs-5840R-FITC)?

FITC on bs-5840R-FITC: excitation / emission 494nm/518nm.