ADAM28 Polyclonal Antibody, Cy7 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-5854R-Cy7 |
| Product Name | ADAM28 Polyclonal Antibody, Cy7 Conjugated |
| Applications |
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat |
| Specifications | |
| Conjugation | Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human ADAM28 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 10863 |
| Swiss Prot | Q9UKQ2 |
| Subcellular location | Secreted, Cell membrane |
| Synonyms | MDCL; MDC-L; eMDCII; ADAM 28; eMDC II; Disintegrin and metalloproteinase domain-containing protein 28; Epididymal metalloproteinase-like, disintegrin-like, and cysteine-rich protein II; Metalloproteinase-like, disintegrin-like, and cysteine-rich protein L; ADAM28; ADAM23 |
| Background | May play a role in the adhesive and proteolytic events that occur during lymphocyte emigration or may function in ectodomain shedding of lymphocyte surface target proteins, such as FASL and CD40L. May be involved in sperm maturation. |
| Application Dilution | |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is ADAM28 Polyclonal Antibody, Cy7 Conjugated (bs-5854R-Cy7) validated for, and at what dilution?
bs-5854R-Cy7 is validated for FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does ADAM28 Polyclonal Antibody, Cy7 Conjugated (bs-5854R-Cy7) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat.
How should bs-5854R-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ADAM28 Polyclonal Antibody, Cy7 Conjugated (bs-5854R-Cy7)?
KLH conjugated synthetic peptide derived from human ADAM28
What are the excitation and emission wavelengths of the Cy7 conjugate (bs-5854R-Cy7)?
Cy7 on bs-5854R-Cy7: excitation / emission 743nm/767nm.