MUC13 Polyclonal Antibody

$Lane 1: Human HeLa cell lysates; Lane 2: Human HepG2 cell lysates; Lane 3: Human A549 cell lysates probed with MUC13 Polyclonal Antibody, Unconjugated (bs-5877R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.$

Lane 1: Human HeLa cell lysates; Lane 2: Human HepG2 cell lysates; Lane 3: Human A549 cell lysates probed with MUC13 Polyclonal Antibody, Unconjugated (bs-5877R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.

$Paraformaldehyde-fixed, paraffin embedded (rat kidney\uff09; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (MUC13) Polyclonal Antibody, Unconjugated (bs-5877R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.$

Paraformaldehyde-fixed, paraffin embedded (rat kidney\uff09; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (MUC13) Polyclonal Antibody, Unconjugated (bs-5877R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Applications

WB
IHC-P
IF(IHC-P)

Reactivity

Human
Mouse
Rat
Pig

Overview
Catalog #	bs-5877r
Product Name	MUC13 Polyclonal Antibody
Applications	WB, IHC-P, IF(IHC-P)
Reactivity	Human, Mouse, Rat, Pig
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human MUC13
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	56667
Subcellular location	Extracellular
Synonyms	MUC13 Down regulated in colon cancer 1; DRCC1; MUC 13; MUC-13; Mucin 13, cell surface associated; RECC; UNQ6194; MUC13_HUMAN.
Background	MUC13 is a mucin. These are secreted or transmembrane glycoproteins that are expressed mainly in the digestive tract and on epithelial surfaces. MUC13 is upregulated in gastric cancer. Mucins have also been implicated in inflammatory bowel diseases.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IF(IHC-P)	1:50-200