Hyaluronidase3 Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-5889R-Cy3 |
| Product Name | Hyaluronidase3 Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Sheep, Pig |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Hyaluronidase3 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 8372 |
| Subcellular location | Cytoplasm, Secreted |
| Synonyms | Hyaluronidase 3; HYAL3; Hyaluronidase-3; Hyaluronoglucosaminidase 3; LUCA14; LUCA3; Minna14; HYAL3_HUMAN. |
| Background | HYAL3 is a protein which is similar in structure to hyaluronidases. Hyaluronidases intracellularly degrade hyaluronan, one of the major glycosaminoglycans of the extracellular matrix. Hyaluronan is thought to be involved in cell proliferation, migration and differentiation. However, this protein has not yet been shown to have hyaluronidase activity. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Hyaluronidase3 Polyclonal Antibody, Cy3 Conjugated (bs-5889R-Cy3) validated for, and at what dilution?
bs-5889R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Hyaluronidase3 Polyclonal Antibody, Cy3 Conjugated (bs-5889R-Cy3) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Sheep, Pig.
How should bs-5889R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Hyaluronidase3 Polyclonal Antibody, Cy3 Conjugated (bs-5889R-Cy3)?
KLH conjugated synthetic peptide derived from human Hyaluronidase3
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-5889R-Cy3)?
Cy3 on bs-5889R-Cy3: excitation / emission 512,550nm/570,615nm.