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Hyaluronidase3 Polyclonal Antibody, HRP Conjugated

Applications

  • WB
  • ELISA
  • IHC-P
  • IHC-F

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Sheep
  • Pig
Overview
Catalog # bs-5889R-HRP
Product Name Hyaluronidase3 Polyclonal Antibody, HRP Conjugated
Applications
WB ELISA IHC-P IHC-F
Reactivity Human, Mouse, Rat
Predicted Reactivity Sheep, Pig
Specifications
Conjugation HRP
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Hyaluronidase3
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 8372
Subcellular location Cytoplasm, Secreted
Synonyms Hyaluronidase 3; HYAL3; Hyaluronidase-3; Hyaluronoglucosaminidase 3; LUCA14; LUCA3; Minna14; HYAL3_HUMAN.
Background HYAL3 is a protein which is similar in structure to hyaluronidases. Hyaluronidases intracellularly degrade hyaluronan, one of the major glycosaminoglycans of the extracellular matrix. Hyaluronan is thought to be involved in cell proliferation, migration and differentiation. However, this protein has not yet been shown to have hyaluronidase activity.
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500

Frequently asked questions

Which applications is Hyaluronidase3 Polyclonal Antibody, HRP Conjugated (bs-5889R-HRP) validated for, and at what dilution?

bs-5889R-HRP is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Hyaluronidase3 Polyclonal Antibody, HRP Conjugated (bs-5889R-HRP) react with?

Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Sheep, Pig.

How should bs-5889R-HRP be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Hyaluronidase3 Polyclonal Antibody, HRP Conjugated (bs-5889R-HRP)?

KLH conjugated synthetic peptide derived from human Hyaluronidase3