HIF Prolyl Hydroxylases Polyclonal Antibody, Biotin Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-5899R-Biotin-100uL |
| Product Name | HIF Prolyl Hydroxylases Polyclonal Antibody, Biotin Conjugated |
| Applications |
WB
ELISA
IHC-P
IHC-F
|
| Predicted Reactivity | Human, Mouse, Rat, Pig, Horse |
| Specifications | |
| Conjugation | Biotin |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human HIFPH4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C for 12 months. |
| Target | |
| Subcellular location | Cytoplasm |
| Synonyms | EGLN4; H prolyl hydroxylase PH4; H-PH4; H-prolyl hydroxylase 4; HPH4; HPH-4; Hypoxia inducible factor prolyl 4 hydroxylase; Hypoxia inducible factor prolyl hydroxylase; Hypoxia-inducible factor prolyl hydroxylase 4; P4H TM; P4H with transmembrane domain; P4H-TM; P4htm; P4HTM_HUMAN; PH 4; PH4; PHD4; Proline 4 hydroxylase; Prolyl 4 hydroxylase transmembrane endoplasmic reticulum; Prolyl hydroxlase domain containing 4; Transmembrane prolyl 4-hydroxylase. |
| Background | Catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF1A at 'Pro-402' and 'Pro-564'. May function as a cellular oxygen sensor and, under normoxic conditions, may target HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
Frequently asked questions
Which applications is HIF Prolyl Hydroxylases Polyclonal Antibody, Biotin Conjugated (bs-5899R-Biotin) validated for, and at what dilution?
bs-5899R-Biotin is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-5899R-Biotin be stored?
Store at -20C for 12 months. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise HIF Prolyl Hydroxylases Polyclonal Antibody, Biotin Conjugated (bs-5899R-Biotin)?
KLH conjugated synthetic peptide derived from human HIFPH4