HIF Prolyl Hydroxylases Polyclonal Antibody, Cy5 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-5899R-Cy5-100uL |
| Product Name | HIF Prolyl Hydroxylases Polyclonal Antibody, Cy5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Pig, Horse |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human HIFPH4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Cytoplasm |
| Synonyms | EGLN4; H prolyl hydroxylase PH4; H-PH4; H-prolyl hydroxylase 4; HPH4; HPH-4; Hypoxia inducible factor prolyl 4 hydroxylase; Hypoxia inducible factor prolyl hydroxylase; Hypoxia-inducible factor prolyl hydroxylase 4; P4H TM; P4H with transmembrane domain; P4H-TM; P4htm; P4HTM_HUMAN; PH 4; PH4; PHD4; Proline 4 hydroxylase; Prolyl 4 hydroxylase transmembrane endoplasmic reticulum; Prolyl hydroxlase domain containing 4; Transmembrane prolyl 4-hydroxylase. |
| Background | Catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF1A at 'Pro-402' and 'Pro-564'. May function as a cellular oxygen sensor and, under normoxic conditions, may target HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is HIF Prolyl Hydroxylases Polyclonal Antibody, Cy5 Conjugated (bs-5899R-Cy5) validated for, and at what dilution?
bs-5899R-Cy5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-5899R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise HIF Prolyl Hydroxylases Polyclonal Antibody, Cy5 Conjugated (bs-5899R-Cy5)?
KLH conjugated synthetic peptide derived from human HIFPH4
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-5899R-Cy5)?
Cy5 on bs-5899R-Cy5: excitation / emission 625,650nm/670nm.