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OS9 Polyclonal Antibody, Cy5.5 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Mouse

Predicted Reactivity

  • Human
  • Rat
  • Dog
  • Cow
  • Sheep
  • Horse
  • Rabbit
Overview
Catalog # bs-5901R-Cy5.5-100uL
Product Name OS9 Polyclonal Antibody, Cy5.5 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Mouse
Predicted Reactivity Human, Rat, Dog, Cow, Sheep, Horse, Rabbit
Specifications
Conjugation Cy5.5
Host Rabbit
Source KLH conjugated synthetic peptide derived from human OS9
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 10956
Subcellular location Cytoplasm
Synonyms lied in osteosarcoma 9; amplied in osteosarcoma; OS-9; Os9; OS9_HUMAN; Protein OS-9.
Background Lectin which functions in endoplasmic reticulum (ER) quality control and ER-associated degradation (ERAD). May bind terminally misfolded non-glycosylated proteins as well as improperly folded glycoproteins, retain them in the ER, and possibly transfer them to the ubiquitination machinery and promote their degradation. Possible targets include TRPV4.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is OS9 Polyclonal Antibody, Cy5.5 Conjugated (BS-5901R-CY5-5) validated for, and at what dilution?

BS-5901R-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does OS9 Polyclonal Antibody, Cy5.5 Conjugated (BS-5901R-CY5-5) react with?

Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow, Sheep, Horse, Rabbit.

How should BS-5901R-CY5-5 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise OS9 Polyclonal Antibody, Cy5.5 Conjugated (BS-5901R-CY5-5)?

KLH conjugated synthetic peptide derived from human OS9

What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-5901R-CY5-5)?

Cy5.5 on BS-5901R-CY5-5: excitation / emission 675nm/694nm.