Ferritin Heavy Chain/FTH1 Polyclonal Antibody, Cy5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-5907R-Cy5 |
| Product Name | Ferritin Heavy Chain/FTH1 Polyclonal Antibody, Cy5 Conjugated |
| Applications |
WB
FCM
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Cow, Sheep, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Ferritin heavy chain |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 2495 |
| Swiss Prot | P02794 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | FHC; FTH; HFE5; PLIF; FTHL6; PIG15; Ferritin heavy chain; Ferritin H subunit; Cell proliferation-inducing gene 15 protein; FTH1; OK/SW-cl.84 |
| Background | Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney (By similarity). |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
Frequently asked questions
Which applications is Ferritin Heavy Chain/FTH1 Polyclonal Antibody, Cy5 Conjugated (bs-5907R-Cy5) validated for, and at what dilution?
bs-5907R-Cy5 is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Ferritin Heavy Chain/FTH1 Polyclonal Antibody, Cy5 Conjugated (bs-5907R-Cy5) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Sheep, Pig, Horse, Rabbit.
How should bs-5907R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Ferritin Heavy Chain/FTH1 Polyclonal Antibody, Cy5 Conjugated (bs-5907R-Cy5)?
KLH conjugated synthetic peptide derived from human Ferritin heavy chain
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-5907R-Cy5)?
Cy5 on bs-5907R-Cy5: excitation / emission 625,650nm/670nm.