Carbonic anhydrase 3 Polyclonal Antibody
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-5914R |
| Product Name | Carbonic anhydrase 3 Polyclonal Antibody |
| Applications |
WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Cow, Pig, Horse |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Carbonic anhydrase 3 |
| Immunogen Range | 21-120/260 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 761 |
| Subcellular location | Cytoplasm |
| Synonyms | CA III; CAIII; Car3; Carbonate dehydratase III; Carbonic anhydrase 3; Carbonic anhydrase3; Carbonic anhydrase-3; Carbonic anhydrase III muscle specic; EC 4.2.1.1. |
| Background | Carbonic anhydrase is a family of metalloenzymes (enzymes that contain one or more metal atoms as a functional component of the enzyme) that catalyze the rapid interconversion of carbon dioxide and water into carbonic acid and protons. It also converts bicarbonate in carbon dioxide and water. This reaction occurs spontaneously in aqueous solution, but does so slowly. Carbonic anhydrase can greatly increase the rate of the reaction, with typical catalytic rates of the different forms of this enzyme ranging between 104 and 106 reactions per second. The active site of most carbonic anhydrases contains a zinc ion. |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Carbonic anhydrase 3 Polyclonal Antibody (bs-5914R) validated for, and at what dilution?
bs-5914R is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-5914R be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Carbonic anhydrase 3 Polyclonal Antibody (bs-5914R)?
KLH conjugated synthetic peptide derived from human Carbonic anhydrase 3 Immunogen range: 21-120/260.