Help

FGF6 Polyclonal Antibody, Cy7 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Dog

Predicted Reactivity

  • Human
  • Mouse
  • Rat
  • Cow
  • Horse
  • Rabbit
Overview
Catalog # bs-5923R-Cy7-100uL
Product Name FGF6 Polyclonal Antibody, Cy7 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Dog
Predicted Reactivity Human, Mouse, Rat, Cow, Horse, Rabbit
Specifications
Conjugation Cy7
Host Rabbit
Source KLH conjugated synthetic peptide derived from human FGF6
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 2251
Subcellular location Secreted, Extracellular matrix
Synonyms FGF 6; FGF6; FGF-6; Fibroblast growth factor 6; Fibroblast growth factor 6 precursor; HBGF 6; HBGF6; HST 2; HST2.
Background FGF6 is a secreted heparin binding growth factor that is a member of the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF6 is expressed in leukemia cell lines with platelet megakaryocytic differentiation potential. It signals through FGFR 1c, 2c, and 4. The FGF6 gene displays oncogenic transforming activity when transfected into mammalian cells. The mouse homolog of this gene exhibits a restricted expression profile predominantly in the myogenic lineage, which suggested a role in muscle regeneration or differentiation.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is FGF6 Polyclonal Antibody, Cy7 Conjugated (bs-5923R-Cy7) validated for, and at what dilution?

bs-5923R-Cy7 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does FGF6 Polyclonal Antibody, Cy7 Conjugated (bs-5923R-Cy7) react with?

Tested reactivity: Dog. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Rat, Cow, Horse, Rabbit.

How should bs-5923R-Cy7 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise FGF6 Polyclonal Antibody, Cy7 Conjugated (bs-5923R-Cy7)?

KLH conjugated synthetic peptide derived from human FGF6

What are the excitation and emission wavelengths of the Cy7 conjugate (bs-5923R-Cy7)?

Cy7 on bs-5923R-Cy7: excitation / emission 743nm/767nm.