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LIMS1 Polyclonal Antibody, HRP Conjugated

Applications

  • ELISA
  • IHC-P
  • IHC-F

Predicted Reactivity

  • Human
  • Mouse
  • Rat
  • Cow
  • Pig
  • Chicken
Overview
Catalog # bs-5951R-HRP-100uL
Product Name LIMS1 Polyclonal Antibody, HRP Conjugated
Applications
ELISA IHC-P IHC-F
Predicted Reactivity Human, Mouse, Rat, Cow, Pig, Chicken
Specifications
Conjugation HRP
Host Rabbit
Source KLH conjugated synthetic peptide derived from human LIMS1
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 3987
Subcellular location Cytoplasm, Cell membrane
Synonyms Lims1l; LIM and senescent cell antigen like domains 1; Particularly interesting new Cys His protein 1; PINCH; PINCH1; Renal carcinoma antigen NY REN 48; LIMS1_HUMAN.
Background Effector of integrin and growth factor signaling, coupling surface receptors to downstream signaling molecules involved in the regulation of cell survival, cell proliferation and cell differentiation. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway.
Application Dilution
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500

Frequently asked questions

Which applications is LIMS1 Polyclonal Antibody, HRP Conjugated (bs-5951R-HRP) validated for, and at what dilution?

bs-5951R-HRP is validated for ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

How should bs-5951R-HRP be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise LIMS1 Polyclonal Antibody, HRP Conjugated (bs-5951R-HRP)?

KLH conjugated synthetic peptide derived from human LIMS1