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AARE Polyclonal Antibody, APC Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse

Predicted Reactivity

  • Rat
  • Cow
  • Sheep
  • Pig
  • Horse
  • Rabbit
Overview
Catalog # bs-5983R-APC-100uL
Product Name AARE Polyclonal Antibody, APC Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Mouse
Predicted Reactivity Rat, Cow, Sheep, Pig, Horse, Rabbit
Specifications
Conjugation APC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human AARE
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 327
Subcellular location Nucleus
Synonyms AARE; ACPH; Acyl peptide hydrolase; Acylamino acid releasing enzyme; Acylaminoacyl peptidase; APEH; APH; D3F15S2; D3S48E; DNF15S2; N acylaminoacyl peptide hydrolase; OPH; Oxidized protein hydrolase; ACPH_HUMAN.
Background AARE (Acylamino-acid-releasing enzyme) is also known as Acyl-peptide hydrolase. It catalyzes the hydrolysis of the terminal acetylated amino acid preferentially from small acetylated peptides. The acetyl amino acid formed by this hydrolase is further processed to acetate and a free amino acid by an aminoacylase. It can play an important role in destroying oxidatively damaged proteins in living cells. Deletions of this gene locus corresponding to the protein are found in various types of carcinomas, including small cell lung carcinoma and renal cell carcinoma.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is AARE Polyclonal Antibody, APC Conjugated (bs-5983R-APC) validated for, and at what dilution?

bs-5983R-APC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does AARE Polyclonal Antibody, APC Conjugated (bs-5983R-APC) react with?

Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Cow, Sheep, Pig, Horse, Rabbit.

How should bs-5983R-APC be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise AARE Polyclonal Antibody, APC Conjugated (bs-5983R-APC)?

KLH conjugated synthetic peptide derived from human AARE

What are the excitation and emission wavelengths of the APC conjugate (bs-5983R-APC)?

APC on bs-5983R-APC: excitation / emission 650nm/660nm.

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