AIM2 Polyclonal Antibody, APC-Cy7 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-5986R-APC-Cy7-100uL |
| Product Name | AIM2 Polyclonal Antibody, APC-Cy7 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | APC-Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from mouse AIM2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 383619 |
| Swiss Prot | Q91VJ1 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | Gm1313; Ifi210; Interferon-inducible protein AIM2; Interferon-inducible protein 210; Ifi-210; Interferon-inducible protein p210; Aim2 |
| Background | Involved in innate immune response by recognizing cytosolic double-stranded DNA and inducing caspase-1-activating inflammasome formation in macrophages. Upon binding to DNA is thought to undergo oligomerization and to associate with PYCARD initiating the recruitment of caspase-1 precusrsor and processing of interleukin-1 beta and interleukin-18. Detects cytosolic dsDNA of viral and bacterial origin in a non-sequence-specific manner. Can also trigger PYCARD-dependent, caspase-1-independent cell death that involves caspase-8. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is AIM2 Polyclonal Antibody, APC-Cy7 Conjugated (bs-5986R-APC-Cy7) validated for, and at what dilution?
bs-5986R-APC-Cy7 is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does AIM2 Polyclonal Antibody, APC-Cy7 Conjugated (bs-5986R-APC-Cy7) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-5986R-APC-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise AIM2 Polyclonal Antibody, APC-Cy7 Conjugated (bs-5986R-APC-Cy7)?
KLH conjugated synthetic peptide derived from mouse AIM2
What are the excitation and emission wavelengths of the APC-Cy7 conjugate (bs-5986R-APC-Cy7)?
APC-Cy7 on bs-5986R-APC-Cy7: excitation / emission 650nm/780nm.