CDC4 Polyclonal Antibody, Cy7 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-5996R-Cy7 |
| Product Name | CDC4 Polyclonal Antibody, Cy7 Conjugated |
| Applications |
IF
|
| Reactivity | Human, Mouse, Rat, Bovine, Dog, Pig |
| Specifications | |
| Conjugation | Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human FBW7 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 55294 |
| Synonyms | AGO; Archipelago homolog; Archipelago, Drosophila, homolog of antibody CDC4; DKFZp686F23254; F box and WD 40 domain protein 7 archipelago homolog, Drosophila; F box and WD 40 domain protein 7; F box and WD repeat domain containing 7; F box protein FBW7; F box protein FBX30; F box protein SEL10; F-box and WD-40 domain-containing protein 7; F-box protein FBX30; F-box/WD repeat-containing protein 7; FBW6; FBW7; FBX30; FBXO30; FBXW6; FBXW7; FBXW7_HUMAN; FLJ16457; hAgo; hCdc4; Homolog of C elegans sel 10; Homolog of C.elegans sel10; SEL-10; SEL10. |
| Background | Fbw7 is a member of the F box protein family which are characterized by an approximately 40 amino acid motif, the F box. The F box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F box), which function in phosphorylation-dependent ubiquitination. The F box proteins are divided into 3 classes: Fbws containing WD40 domains, Fbls containing leucine rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. Fbw7 belongs to the Fbws class; in addition to an F box, this protein contains 7 tandem WD40 repeats. It binds directly to cyclin E and probably targets cyclin E for ubiquitin mediated degradation. Mutations of this gene are detected in ovarian and breast cancer cell lines. Alternative splicing of this gene generates 2 transcript variants diverging at the 5' termini. |
| Application Dilution | |
| IF | |
Frequently asked questions
Which applications is CDC4 Polyclonal Antibody, Cy7 Conjugated (bs-5996R-Cy7) validated for, and at what dilution?
bs-5996R-Cy7 is validated for IF.
Which species does CDC4 Polyclonal Antibody, Cy7 Conjugated (bs-5996R-Cy7) react with?
Tested reactivity: Human, Mouse, Rat, Bovine, Dog, Pig.
How should bs-5996R-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CDC4 Polyclonal Antibody, Cy7 Conjugated (bs-5996R-Cy7)?
KLH conjugated synthetic peptide derived from human FBW7