Contact us at 1.800.501.7654 or info@biossusa.com

MenuSearch
Help

Acetyl-Histone H4 (Lys16) Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Acetyl-Histone H4 (Lys16) Polyclonal Antibody, Unconjugated (bs-60036R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Acetyl-Histone H4 (Lys16) Polyclonal Antibody, Unconjugated (bs-60036R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Acetyl-Histone H4 (Lys16) Polyclonal Antibody, Unconjugated (bs-60036R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Acetyl-Histone H4 (Lys16) Polyclonal Antibody, Unconjugated (bs-60036R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Acetyl-Histone H4 (Lys16) Polyclonal Antibody, Unconjugated (bs-60036R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Acetyl-Histone H4 (Lys16) Polyclonal Antibody, Unconjugated (bs-60036R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Acetyl-Histone H4 (Lys16) Polyclonal Antibody, Unconjugated (bs-60036R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Acetyl-Histone H4 (Lys16) Polyclonal Antibody, Unconjugated (bs-60036R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Acetyl-Histone H4 (Lys16) Polyclonal Antibody, Unconjugated (bs-60036R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Acetyl-Histone H4 (Lys16) Polyclonal Antibody, Unconjugated (bs-60036R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Chromatin immunoprecipitation was performed using cross-linked chromatin from 5\u00d710\u2076 HeLa cells per IP, with 5 \u03bcg of bs-60036R antibody or 5 \u03bcg of normal rabbit IgG(negative control bs-0295P), and 20 \u03bcL of Protein A\/G MagBeads per IP. The immunoprecipitated DNA was quantified by real-time PCR using primers specific for the RPL30 exon 4 and human GAPDH-CDS regions. The data are presented as enrichment of each sample relative to the total amount of input at each amplicon.
Chromatin immunoprecipitation was performed using cross-linked chromatin from 5\u00d710\u2076 HeLa cells per IP, with 5 \u03bcg of bs-60036R antibody or 5 \u03bcg of normal rabbit IgG(negative control bs-0295P), and 20 \u03bcL of Protein A\/G MagBeads per IP. The immunoprecipitated DNA was quantified by real-time PCR using primers specific for the RPL30 exon 4 and human GAPDH-CDS regions. The data are presented as enrichment of each sample relative to the total amount of input at each amplicon.
4% Paraformaldehyde-fixed HeLa (treated with Trichostatin A (TSA)(1\u03bcM, 18h)) (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (Histone H4 (acetyl K16)) polyclonal Antibody, unconjugated (bs-60036R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
4% Paraformaldehyde-fixed HeLa (treated with Trichostatin A (TSA)(1\u03bcM, 18h)) (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (Histone H4 (acetyl K16)) polyclonal Antibody, unconjugated (bs-60036R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
The HeLa (treated with Trichostatin A (TSA)(1\u03bcM, 18h)) (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-Histone H4 (acetyl K16) antibody (bs-60036R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
The HeLa (treated with Trichostatin A (TSA)(1\u03bcM, 18h)) (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-Histone H4 (acetyl K16) antibody (bs-60036R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
25 ug total protein per lane of various lysates (see on figure) probed with Histone H4 (acetyl K16) polyclonal antibody, unconjugated (bs-60036R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with Histone H4 (acetyl K16) polyclonal antibody, unconjugated (bs-60036R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Bioss unconjugated antibody vial

Applications

  • WB
  • IHC-P
  • IHC-F
  • IF
  • ICC/IF

Reactivity

  • Human
  • Mouse
  • Rat
  • Rice
Overview
Catalog # bs-60036r
Product Name Acetyl-Histone H4 (Lys16) Polyclonal Antibody
Applications
WB IHC-P IHC-F IF ICC/IF
Reactivity Human, Mouse, Rat, Rice
Specifications
Conjugation Unconjugated
Host Rabbit
Clonality Polyclonal
Isotype IgG
Concentration Lot Dependent
Purification Protein A coupled with the antigen peptide containing acetylated H4 (Lys16).
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Swiss Prot P62805
Application Dilution
WB =1:500-1:2000
IHC-P IHC-P=1:100-500
IHC-F IHC-F=1:100-500
IF IF=0
ICC/IF ICC/IF=1:50-1:200

Frequently asked questions

Which applications is Acetyl-Histone H4 (Lys16) Polyclonal Antibody (bs-60036R) validated for, and at what dilution?

bs-60036R is validated for WB (WB=1:500-1:2000); IHC-P (IHC-P=1:100-500); IHC-F (IHC-F=1:100-500); IF (IF=0); ICC/IF (ICC/IF=1:50-1:200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Acetyl-Histone H4 (Lys16) Polyclonal Antibody (bs-60036R) react with?

Tested reactivity: Human, Mouse, Rat, Rice.

How should bs-60036R be stored?

Store at -20°C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Search our catalog of hundreds of thousands of products