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DAL1/EPB41L3 Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with DAL1 Polyclonal Antibody, Unconjugated (bs-6003R) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with DAL1 Polyclonal Antibody, Unconjugated (bs-6003R) at 1:400 overnight at 4\u00b0C, DAB staining.
Lane 1: Human HUVEC cell lysates; Lane 2: Human U-2 OS cell lysates probed with DAL1\/EPB41L3 Polyclonal Antibody, Unconjugated (bs-6003R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.
Lane 1: Human HUVEC cell lysates; Lane 2: Human U-2 OS cell lysates probed with DAL1\/EPB41L3 Polyclonal Antibody, Unconjugated (bs-6003R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.
Bioss unconjugated antibody vial

Applications

  • WB
  • IHC-P
  • IHC-F
  • IF

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Dog
  • Cow
  • Sheep
  • Pig
  • Horse
  • Chicken
  • Rabbit
Overview
Catalog # bs-6003R
Product Name DAL1/EPB41L3 Polyclonal Antibody
Applications
WB IHC-P IHC-F IF
Reactivity Human, Mouse, Rat
Predicted Reactivity Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human DAL1/EPB41L3
Clonality Polyclonal
Isotype IgG
Concentration 1mg/ml
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 23136
Swiss Prot Q9Y2J2
Subcellular location Cell membrane
Synonyms Differentially expressed in adenocarcinoma of the lung protein 1; 4.1B; Band 4.1 like protein 3; DAL 1; DAL1; EPB41L3; Erythrocyte membrane protein band 4.1 like 3; HGNC:3380; KIAA0987; E41L3_HUMAN.
Application Dilution
WB =1:500-2000
IHC-P ELISAELISA=1:5000-10000
IHC-F
IF

Frequently asked questions

Which applications is DAL1/EPB41L3 Polyclonal Antibody (bs-6003R) validated for, and at what dilution?

bs-6003R is validated for WB (WB=1:500-2000); ELISA (ELISA=1:5000-10000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does DAL1/EPB41L3 Polyclonal Antibody (bs-6003R) react with?

Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit.

How should bs-6003R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise DAL1/EPB41L3 Polyclonal Antibody (bs-6003R)?

KLH conjugated synthetic peptide derived from human DAL1/EPB41L3

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