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EXTL2 Polyclonal Antibody, Cy3 Conjugated

Applications

  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Predicted Reactivity

  • Human
  • Mouse
  • Rat
  • Dog
  • Sheep
  • Pig
  • Horse
  • Rabbit
Overview
Catalog # bs-6005R-Cy3
Product Name EXTL2 Polyclonal Antibody, Cy3 Conjugated
Applications
IF(IHC-P) IF(IHC-F) IF(ICC)
Predicted Reactivity Human, Mouse, Rat, Dog, Sheep, Pig, Horse, Rabbit
Specifications
Conjugation Cy3
Host Rabbit
Source KLH conjugated synthetic peptide derived from human EXTL
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 2134
Subcellular location Cytoplasm, Secreted, Cell membrane
Synonyms Alpha 1 4 N acetylhexosaminyltransferase EXTL2; Alpha GalNAcT EXTL2; Exostoses multiple like 2; Exostoses multiple like 2; Exostosin like 2; EXT L2; EXT related protein 2; EXTL 2; EXTR 2; EXTR2; Glucuronyl galactosyl proteoglycan 4 alpha N acetylglucosaminyltransferase; Multiple exostoses like 2; Processed exostosin like 2; EXTL2_HUMAN.
Background EXTL2 is a glycosyltransferase required for the biosynthesis of heparan-sulfate and is responsible for the alternating addition of beta-1-4-linked glucuronic acid (GlcA) and alpha-1-4-linked N-acetylglucosamine (GlcNAc) units to nascent heparan sulfate chains.
Application Dilution
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is EXTL2 Polyclonal Antibody, Cy3 Conjugated (bs-6005R-Cy3) validated for, and at what dilution?

bs-6005R-Cy3 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

How should bs-6005R-Cy3 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise EXTL2 Polyclonal Antibody, Cy3 Conjugated (bs-6005R-Cy3)?

KLH conjugated synthetic peptide derived from human EXTL

What are the excitation and emission wavelengths of the Cy3 conjugate (bs-6005R-Cy3)?

Cy3 on bs-6005R-Cy3: excitation / emission 512,550nm/570,615nm.