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Symmetric Di-Methyl-Histone H3 (Arg8) Polyclonal Antibody, Cy5 Conjugated

Applications

  • WB

Reactivity

  • Human

Predicted Reactivity

  • Mouse
  • Rat
  • Monkey
Overview
Catalog # bs-60147r-cy5-100ul
Product Name Symmetric Di-Methyl-Histone H3 (Arg8) Polyclonal Antibody, Cy5 Conjugated
Applications
WB
Reactivity Human
Predicted Reactivity Mouse, Rat, Monkey
Specifications
Conjugation Cy5
Host Rabbit
Clonality Polyclonal
Isotype IgG
Concentration 1mg/ml
Purification Protein A coupled with the antigen peptide containing H3R8me2 (sy).
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Swiss Prot P68431
Application Dilution
WB 1:500-1:2000

Frequently asked questions

Which applications is Symmetric Di-Methyl-Histone H3 (Arg8) Polyclonal Antibody, Cy5 Conjugated (bs-60147R-Cy5) validated for, and at what dilution?

bs-60147R-Cy5 is validated for WB (1:500-1:2000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Symmetric Di-Methyl-Histone H3 (Arg8) Polyclonal Antibody, Cy5 Conjugated (bs-60147R-Cy5) react with?

Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Monkey.

How should bs-60147R-Cy5 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What are the excitation and emission wavelengths of the Cy5 conjugate (bs-60147R-Cy5)?

Cy5 on bs-60147R-Cy5: excitation / emission 625,650nm/670nm.