Aminoacylase 1 Polyclonal Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-6019R-100uL |
| Product Name | Aminoacylase 1 Polyclonal Antibody |
| Applications |
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Rat |
| Predicted Reactivity | Human, Mouse, Cow, Pig, Horse |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human ACY1/Aminoacylase 1 |
| Immunogen Range | 201-300/408 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 95 |
| Subcellular location | Cytoplasm |
| Synonyms | ACY 1; ACY1; ACY1D; ACYLASE; EC 3.5.1.14; N acyl L amino acid amidohydrolase; ACY1_HUMAN. |
| Background | Aminoacylase 1 is a cytosolic, homodimeric, zinc binding enzyme that catalyzes the hydrolysis of acylated L amino acids to L amino acids and acyl group, and has been postulated to function in the catabolism and salvage of acylated amino acids. ACY1 has been assigned to chromosome 3p21.1, a region reduced to homozygosity in small cell lung cancer (SCLC), and its expression has been reported to be reduced or undetectable in SCLC cell lines and tumors. The amino acid sequence of human aminoacylase 1 is highly homologous to the porcine counterpart, and ACY1 is the first member of a new family of zinc binding enzymes. |
| Application Dilution | |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Aminoacylase 1 Polyclonal Antibody (bs-6019R) validated for, and at what dilution?
bs-6019R is validated for ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Aminoacylase 1 Polyclonal Antibody (bs-6019R) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Cow, Pig, Horse.
How should bs-6019R be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Aminoacylase 1 Polyclonal Antibody (bs-6019R)?
KLH conjugated synthetic peptide derived from human ACY1/Aminoacylase 1 Immunogen range: 201-300/408.