F(ab')₂ Fragment Goat Anti-Mouse IgG (H+L), AbBy Fluor® 405 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-60296g-bf405-200ul |
| Product Name | F(ab')₂ Fragment Goat Anti-Mouse IgG (H+L), AbBy Fluor® 405 Conjugated |
| Applications |
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse |
| Specifications | |
| Conjugation | AbBy Fluor® 405 |
| Host | Goat |
| Source | Native Mouse IgG |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | Lyophilized |
| Purification | Purified by Protein G. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Background | Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants. |
| Application Dilution | |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is F(ab')₂ Fragment Goat Anti-Mouse IgG (H+L), AbBy Fluor® 405 Conjugated (bs-60296G-BF405) validated for, and at what dilution?
bs-60296G-BF405 is validated for FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does F(ab')₂ Fragment Goat Anti-Mouse IgG (H+L), AbBy Fluor® 405 Conjugated (bs-60296G-BF405) react with?
Tested reactivity: Mouse.
How should bs-60296G-BF405 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise F(ab')₂ Fragment Goat Anti-Mouse IgG (H+L), AbBy Fluor® 405 Conjugated (bs-60296G-BF405)?
Native Mouse IgG
What are the excitation and emission wavelengths of the AbBy Fluor® 405 conjugate (bs-60296G-BF405)?
AbBy Fluor® 405 on bs-60296G-BF405: excitation / emission 401nm/421nm.