phospho-CD32 (Tyr292) Polyclonal Antibody, FITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-6031R-FITC-100uL |
| Product Name | phospho-CD32 (Tyr292) Polyclonal Antibody, FITC Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Monkey |
| Predicted Reactivity | Rat, Dog, Sheep, Pig, Rabbit, Guinea Pig |
| Specifications | |
| Conjugation | FITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human CD32B around the phosphorylation site of Tyr292. |
| Modification Site | Tyr292 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 2213 |
| Swiss Prot | P31994 |
| Subcellular location | Cell membrane |
| Synonyms | CD32; FCG2; CD32B; FCGR2; IGFR2; Low affinity immunoglobulin gamma Fc region receptor II-b; IgG Fc receptor II-b; CDw32; Fc-gamma RII-b; Fc-gamma-RIIb; FcRII-b; FCGR2B |
| Background | Receptor for the Fc region of complexed or aggregated immunoglobulins gamma. Low affinity receptor. Involved in a variety of effector and regulatory functions such as phagocytosis of immune complexes and modulation of antibody production by B-cells. Binding to this receptor results in down-modulation of previous state of cell activation triggered via antigen receptors on B-cells (BCR), T-cells (TCR) or via another Fc receptor. Isoform IIB1 fails to mediate endocytosis or phagocytosis. Isoform IIB2 does not trigger phagocytosis. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-CD32 (Tyr292) Polyclonal Antibody, FITC Conjugated (bs-6031R-FITC) validated for, and at what dilution?
bs-6031R-FITC is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-CD32 (Tyr292) Polyclonal Antibody, FITC Conjugated (bs-6031R-FITC) react with?
Tested reactivity: Human, Mouse, Monkey. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Dog, Sheep, Pig, Rabbit, Guinea Pig.
How should bs-6031R-FITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-CD32 (Tyr292) Polyclonal Antibody, FITC Conjugated (bs-6031R-FITC)?
KLH conjugated synthetic phosphopeptide derived from human CD32B around the phosphorylation site of Tyr292.
What are the excitation and emission wavelengths of the FITC conjugate (bs-6031R-FITC)?
FITC on bs-6031R-FITC: excitation / emission 494nm/518nm.