Protective protein/Cathepsin A Polyclonal Antibody

Formalin-fixed and paraffin embedded rat liver tissue labeled with Anti Protective Protein Polyclonal Antibody, Unconjugated (bs-6040R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Mouse testis lysates probed withRabbit Anti-Lysosomal Protective Protein Polyclonal Antibody, Unconjugated (bs-6040R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:5000 for 90 min at 37˚C.

$Human 293T lysates probed with Lysosomal Protective Protein Polyclonal Antibody, unconjugated (bs-6040R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.$

Human 293T lysates probed with Lysosomal Protective Protein Polyclonal Antibody, unconjugated (bs-6040R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.

Applications

WB
ELISA
FCM
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Dog
Cow
Pig
Horse

Overview
Catalog #	bs-6040R
Product Name	Protective protein/Cathepsin A Polyclonal Antibody
Applications	WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Dog, Cow, Pig, Horse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Lysosomal Protective Protein
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	5476
Swiss Prot	P10619
Subcellular location	Cytoplasm
Synonyms	GSL; GLB2; NGBE; PPCA; PPGB; Lysosomal protective protein; Carboxypeptidase C; Carboxypeptidase L; Cathepsin A; Protective protein cathepsin A; Protective protein for beta-galactosidase; CTSA
Background	Protective protein appears to be essential for both the activity of beta-galactosidase and neuraminidase, it associates with these enzymes and exerts a protective function necessary for their stability and activity. This protein is also a carboxypeptidase and can deamidate tachykinins.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
FCM	1:20-100
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200