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NARC1/PCSK9 Polyclonal Antibody

Formalin-fixed and paraffin embedded mouse brain labeled with Rabbit Anti-NARC1 Polyclonal Antibody, Unconjugated (bs-6060R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
Formalin-fixed and paraffin embedded mouse brain labeled with Rabbit Anti-NARC1 Polyclonal Antibody, Unconjugated (bs-6060R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with NARC1 Polyclonal Antibody (bs-6060R) at 1:400 overnight at 4\u00b0C, followed by a SP kit and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with NARC1 Polyclonal Antibody (bs-6060R) at 1:400 overnight at 4\u00b0C, followed by a SP kit and DAB staining.
Lane 1: Human A431 cell lysates; Lane 2: Mouse Uterus lysates probed with NARC1 Polyclonal Antibody, Unconjugated (bs-6060R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.
Lane 1: Human A431 cell lysates; Lane 2: Mouse Uterus lysates probed with NARC1 Polyclonal Antibody, Unconjugated (bs-6060R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.
Lane 1: Human HepG2 cell lysates; Lane 2: Human SW480 cell lysates probed with NARC1 Polyclonal Antibody, Unconjugated (bs-6060R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.
Lane 1: Human HepG2 cell lysates; Lane 2: Human SW480 cell lysates probed with NARC1 Polyclonal Antibody, Unconjugated (bs-6060R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.
K562 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with NARC1 Polyclonal Antibody(bs-6060R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
K562 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with NARC1 Polyclonal Antibody(bs-6060R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (NARC1) Polyclonal Antibody, Unconjugated (bs-6060R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (NARC1) Polyclonal Antibody, Unconjugated (bs-6060R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Lane 1: Recombinant human PCSK9 protein, C-His (HEK293)( bs-43105P) probed with NARC1\/PCSK9 Polyclonal Antibody, Unconjugated (bs-6060R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.
Lane 1: Recombinant human PCSK9 protein, C-His (HEK293)( bs-43105P) probed with NARC1\/PCSK9 Polyclonal Antibody, Unconjugated (bs-6060R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.
Bioss unconjugated antibody vial

Applications

  • WB
  • ELISA
  • IHC-P
  • IHC-F
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bs-6060R-100uL
Product Name NARC1/PCSK9 Polyclonal Antibody
Applications
WB ELISA IHC-P IHC-F IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human PCSK9/NARC1
Immunogen Range 231-330/692
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 255738
Swiss Prot Q8NBP7
Subcellular location Secreted
Synonyms FH3; PC9; NARC1; LDLCQ1; NARC-1; HCHOLA3; Proprotein convertase subtilisin/kexin type 9; Neural apoptosis-regulated convertase 1; Proprotein convertase 9; Subtilisin/kexin-like protease PC9; PCSK9; PSEC0052
Background Crucial player in the regulation of plasma cholesterol homeostasis. Binds to low-density lipid receptor family members: low density lipoprotein receptor (LDLR), very low density lipoprotein receptor (VLDLR), apolipoprotein E receptor (LRP1/APOER) and apolipoprotein receptor 2 (LRP8/APOER2), and promotes their degradation in intracellular acidic compartments (PubMed:18039658). Acts via a non-proteolytic mechanism to enhance the degradation of the hepatic LDLR through a clathrin LDLRAP1/ARH-mediated pathway. May prevent the recycling of LDLR from endosomes to the cell surface or direct it to lysosomes for degradation. Can induce ubiquitination of LDLR leading to its subsequent degradation (PubMed:18799458, PubMed:17461796, PubMed:18197702, PubMed:22074827). Inhibits intracellular degradation of APOB via the autophagosome/lysosome pathway in a LDLR-independent manner. Involved in the disposal of non-acetylated intermediates of BACE1 in the early secretory pathway (PubMed:18660751). Inhibits epithelial Na(+) channel (ENaC)-mediated Na(+) absorption by reducing ENaC surface expression primarily by increasing its proteasomal degradation. Regulates neuronal apoptosis via modulation of LRP8/APOER2 levels and related anti-apoptotic signaling pathways.
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is NARC1/PCSK9 Polyclonal Antibody (bs-6060R) validated for, and at what dilution?

bs-6060R is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does NARC1/PCSK9 Polyclonal Antibody (bs-6060R) react with?

Tested reactivity: Human, Mouse, Rat.

How should bs-6060R be stored?

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise NARC1/PCSK9 Polyclonal Antibody (bs-6060R)?

KLH conjugated synthetic peptide derived from human PCSK9/NARC1 Immunogen range: 231-330/692.

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