phospho-LLGL1 + LLGL2 (Ser650+Ser654) Polyclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-6070R-Cy5.5-100uL |
| Product Name | phospho-LLGL1 + LLGL2 (Ser650+Ser654) Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Rat |
| Predicted Reactivity | Human, Mouse, Dog, Cow, Pig, Horse, Chicken, Guinea Pig |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from mouse LLGL1 around the phosphorylation site of Ser650+Ser654 |
| Modification Site | Ser650+Ser654 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3996 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | LLGL1 + LLGL2 phospho S650 + S654; LLGL1 + LLGL2 phospho Ser650 + Ser654; p-LLGL1 + p-LLGL2 S650 + S654; p-LLGL1/LLGL2 Ser650 + Ser654; p-LLGL1/LLGL2 S650 + S654; p-LLGL1/LLGL2 Ser650/654; DLG4; HGL; HUGL; HUGL1; lethal giant larvae homolog 1; lethal giant larvae homolog 2; Lethal2 giant larvae protein homolog 1; Lethal2 giant larvae protein homolog 2; LGL2; LLGL; L2GL1_HUMAN; L2GL2_HUMAN. |
| Background | LLGL1 is a protein that is similar to a tumor suppressor in Drosophila. The protein is part of a cytoskeletal network and is associated with nonmuscle myosin II heavy chain and a kinase that specifically phosphorylates this protein at serine residues. The gene for LLGL1 is located within the Smith-Magenis syndrome region on chromosome 17. LLGL2 is a protein similar to lethal giant larvae of Drosophila. In fly, the protein's ability to localize cell fate determinants is regulated by the atypical protein kinase C (aPKC). In human, this protein interacts with aPKC-containing complexes and is cortically localized in mitotic cells. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-LLGL1 + LLGL2 (Ser650+Ser654) Polyclonal Antibody, Cy5.5 Conjugated (BS-6070R-CY5-5) validated for, and at what dilution?
BS-6070R-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-LLGL1 + LLGL2 (Ser650+Ser654) Polyclonal Antibody, Cy5.5 Conjugated (BS-6070R-CY5-5) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Dog, Cow, Pig, Horse, Chicken, Guinea Pig.
How should BS-6070R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-LLGL1 + LLGL2 (Ser650+Ser654) Polyclonal Antibody, Cy5.5 Conjugated (BS-6070R-CY5-5)?
KLH conjugated synthetic phosphopeptide derived from mouse LLGL1 around the phosphorylation site of Ser650+Ser654
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-6070R-CY5-5)?
Cy5.5 on BS-6070R-CY5-5: excitation / emission 675nm/694nm.