G21 protein/NPR2L Polyclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-6079R-Cy5.5-100uL |
| Product Name | G21 protein/NPR2L Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Rat |
| Predicted Reactivity | Mouse, Cow, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Gene 21 protein/NPRL2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | G21 protein; Gene 21 protein; Homologous to yeast nitrogen permease candidate tumor suppressor; Homologous to yeast nitrogen permease; NPR 2L; NPR L2; NPR like 2; NPR2 like; NPRL 2; NPRL2; Tumor suppressor candidate 4; TUSC 4; TUSC 4 protein; TUSC4; TUSC4 protein; NPRL2_HUMAN. |
| Background | NPR2L is homologous to yeast nitrogen permease and is a candidate tumor suppressor, being a negative regulator of cell cycle. Most abundant in skeletal muscle, followed by brain, liver, and pancreas, with lower amounts in lung, kidney, placenta, and heart. Expressed in most lung cancer cell lines tested. There are two isoforms, produced by alternative splicing. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is G21 protein/NPR2L Polyclonal Antibody, Cy5.5 Conjugated (BS-6079R-CY5-5) validated for, and at what dilution?
BS-6079R-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does G21 protein/NPR2L Polyclonal Antibody, Cy5.5 Conjugated (BS-6079R-CY5-5) react with?
Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Cow, Pig, Horse, Rabbit.
How should BS-6079R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise G21 protein/NPR2L Polyclonal Antibody, Cy5.5 Conjugated (BS-6079R-CY5-5)?
KLH conjugated synthetic peptide derived from human Gene 21 protein/NPRL2
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-6079R-CY5-5)?
Cy5.5 on BS-6079R-CY5-5: excitation / emission 675nm/694nm.