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G21 protein/NPR2L Polyclonal Antibody, Cy7 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Rat

Predicted Reactivity

  • Mouse
  • Cow
  • Pig
  • Horse
  • Rabbit
Overview
Catalog # bs-6079R-Cy7
Product Name G21 protein/NPR2L Polyclonal Antibody, Cy7 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Rat
Predicted Reactivity Mouse, Cow, Pig, Horse, Rabbit
Specifications
Conjugation Cy7
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Gene 21 protein/NPRL2
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Subcellular location Cytoplasm, Cell membrane
Synonyms G21 protein; Gene 21 protein; Homologous to yeast nitrogen permease candidate tumor suppressor; Homologous to yeast nitrogen permease; NPR 2L; NPR L2; NPR like 2; NPR2 like; NPRL 2; NPRL2; Tumor suppressor candidate 4; TUSC 4; TUSC 4 protein; TUSC4; TUSC4 protein; NPRL2_HUMAN.
Background NPR2L is homologous to yeast nitrogen permease and is a candidate tumor suppressor, being a negative regulator of cell cycle. Most abundant in skeletal muscle, followed by brain, liver, and pancreas, with lower amounts in lung, kidney, placenta, and heart. Expressed in most lung cancer cell lines tested. There are two isoforms, produced by alternative splicing.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is G21 protein/NPR2L Polyclonal Antibody, Cy7 Conjugated (bs-6079R-Cy7) validated for, and at what dilution?

bs-6079R-Cy7 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does G21 protein/NPR2L Polyclonal Antibody, Cy7 Conjugated (bs-6079R-Cy7) react with?

Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Cow, Pig, Horse, Rabbit.

How should bs-6079R-Cy7 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise G21 protein/NPR2L Polyclonal Antibody, Cy7 Conjugated (bs-6079R-Cy7)?

KLH conjugated synthetic peptide derived from human Gene 21 protein/NPRL2

What are the excitation and emission wavelengths of the Cy7 conjugate (bs-6079R-Cy7)?

Cy7 on bs-6079R-Cy7: excitation / emission 743nm/767nm.