PHAP1 Polyclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-6083R-Cy5.5-100uL |
| Product Name | PHAP1 Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Specificity | Will recognize Acidic leucine-rich nuclear phosphoprotein 32 family member C,D, and E |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Cow, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human PHAP1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 8125 |
| Swiss Prot | P39687 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | Acidic leucine-rich nuclear phosphoprotein 32 family member A; Acidic nuclear phosphoprotein pp32; pp32; Leucine-rich acidic nuclear protein; LANP; Mapmodulin; Potent heat-stable protein phosphatase 2A inhibitor I1PP2A; Putative HLA-DR-associated protein I; PHAPI; ANP32A; C15orf1; MAPM; PHAP1 |
| Background | Implicated in a number of cellular processes, including proliferation, differentiation, caspase-dependent and caspase-independent apoptosis, suppression of transformation (tumor suppressor), inhibition of protein phosphatase 2A, regulation of mRNA trafficking and stability in association with ELAVL1, and inhibition of acetyltransferases as part of the INHAT (inhibitor of histone acetyltransferases) complex. Plays a role in E4F1-mediated transcriptional repression. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is PHAP1 Polyclonal Antibody, Cy5.5 Conjugated (BS-6083R-CY5-5) validated for, and at what dilution?
BS-6083R-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does PHAP1 Polyclonal Antibody, Cy5.5 Conjugated (BS-6083R-CY5-5) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Pig, Horse, Rabbit.
How should BS-6083R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise PHAP1 Polyclonal Antibody, Cy5.5 Conjugated (BS-6083R-CY5-5)?
KLH conjugated synthetic peptide derived from human PHAP1
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-6083R-CY5-5)?
Cy5.5 on BS-6083R-CY5-5: excitation / emission 675nm/694nm.