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LUCA15 Polyclonal Antibody, HRP Conjugated

Applications

  • WB
  • ELISA
  • IHC-P
  • IHC-F

Reactivity

  • Rat

Predicted Reactivity

  • Human
  • Mouse
  • Dog
  • Cow
  • Pig
  • Horse
  • Rabbit
Overview
Catalog # bs-6090R-HRP
Product Name LUCA15 Polyclonal Antibody, HRP Conjugated
Applications
WB ELISA IHC-P IHC-F
Reactivity Rat
Predicted Reactivity Human, Mouse, Dog, Cow, Pig, Horse, Rabbit
Specifications
Conjugation HRP
Host Rabbit
Source KLH conjugated synthetic peptide derived from human RNA-binding protein 5
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Subcellular location Nucleus
Synonyms G15 antibody H37 antibody LUCA 15; LUCA15; Protein G15; RBM 5; Renal carcinoma antigen NY REN 9; RMB 5; RMB5; RNA binding mot protein 5; RNA binding protein 5; Tumor suppressor LUCA15; RBM5_HUMAN.
Background RBM5 (RNA binding motif protein 5) is a nuclear SR related RNA binding protein which specifically binds poly(G) RNA homopolymers in vitro. It modulates both apoptosis and the cell cycle, and retards tumor formation.
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500

Frequently asked questions

Which applications is LUCA15 Polyclonal Antibody, HRP Conjugated (bs-6090R-HRP) validated for, and at what dilution?

bs-6090R-HRP is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does LUCA15 Polyclonal Antibody, HRP Conjugated (bs-6090R-HRP) react with?

Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Dog, Cow, Pig, Horse, Rabbit.

How should bs-6090R-HRP be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise LUCA15 Polyclonal Antibody, HRP Conjugated (bs-6090R-HRP)?

KLH conjugated synthetic peptide derived from human RNA-binding protein 5