RHOBTB1 Polyclonal Antibody, Cy3 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-6092R-Cy3 |
| Product Name | RHOBTB1 Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Specificity | 84.6% sequence similarity to RHOBTB2 |
| Predicted Reactivity | Human, Mouse, Rat, Cow, Sheep, Horse, Chicken, Rabbit |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human RHOBTB1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 9886 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | Rho related BTB domain containing 1; Rho related BTB domain containing protein 1; RHBT1_HUMAN. |
| Background | RHOBTB1 is a member of the Rho family of the small GTPase superfamily. Is made of a GTPase domain, a proline-rich region, a tandem of 2 BTB (broad complex, tramtrack, and bric-a-brac) domains, and a conserved C-terminal region. Plays a role in small GTPase-mediated signal transduction and the organization of the actin filament system. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is RHOBTB1 Polyclonal Antibody, Cy3 Conjugated (bs-6092R-Cy3) validated for, and at what dilution?
bs-6092R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-6092R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise RHOBTB1 Polyclonal Antibody, Cy3 Conjugated (bs-6092R-Cy3)?
KLH conjugated synthetic peptide derived from human RHOBTB1
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-6092R-Cy3)?
Cy3 on bs-6092R-Cy3: excitation / emission 512,550nm/570,615nm.