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RHOBTB1 Polyclonal Antibody, HRP Conjugated

Applications

  • WB
  • ELISA
  • IHC-P
  • IHC-F

Predicted Reactivity

  • Human
  • Mouse
  • Rat
  • Cow
  • Sheep
  • Horse
  • Chicken
  • Rabbit
Overview
Catalog # bs-6092R-HRP
Product Name RHOBTB1 Polyclonal Antibody, HRP Conjugated
Applications
WB ELISA IHC-P IHC-F
Specificity 84.6% sequence similarity to RHOBTB2
Predicted Reactivity Human, Mouse, Rat, Cow, Sheep, Horse, Chicken, Rabbit
Specifications
Conjugation HRP
Host Rabbit
Source KLH conjugated synthetic peptide derived from human RHOBTB1
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 9886
Subcellular location Cytoplasm, Cell membrane
Synonyms Rho related BTB domain containing 1; Rho related BTB domain containing protein 1; RHBT1_HUMAN.
Background RHOBTB1 is a member of the Rho family of the small GTPase superfamily. Is made of a GTPase domain, a proline-rich region, a tandem of 2 BTB (broad complex, tramtrack, and bric-a-brac) domains, and a conserved C-terminal region. Plays a role in small GTPase-mediated signal transduction and the organization of the actin filament system.
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500

Frequently asked questions

Which applications is RHOBTB1 Polyclonal Antibody, HRP Conjugated (bs-6092R-HRP) validated for, and at what dilution?

bs-6092R-HRP is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

How should bs-6092R-HRP be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise RHOBTB1 Polyclonal Antibody, HRP Conjugated (bs-6092R-HRP)?

KLH conjugated synthetic peptide derived from human RHOBTB1