Rho GTPase activating protein 29 Antibody, AbBy Fluor-405 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-6098R-BF405-100uL |
| Product Name | Rho GTPase activating protein 29 Antibody, AbBy Fluor-405 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Cow, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | AbBy Fluor-405 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human PARG1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 9411 |
| Swiss Prot | Q52LW3 |
| Subcellular location | Cytoplasm |
| Synonyms | PARG1; Rho GTPase-activating protein 29; PTPL1-associated RhoGAP protein 1; Rho-type GTPase-activating protein 29; ARHGAP29 |
| Background | GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state. Has strong activity toward RHOA, and weaker activity toward RAC1 and CDC42. May act as a specific effector of RAP2A to regulate Rho. In concert with RASIP1, suppresses RhoA signaling and dampens ROCK and MYH9 activities in endothelial cells and plays an essential role in blood vessel tubulogenesis. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Rho GTPase activating protein 29 Antibody, AbBy Fluor-405 Conjugated (bs-6098R-BF405) validated for, and at what dilution?
bs-6098R-BF405 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-6098R-BF405 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Rho GTPase activating protein 29 Antibody, AbBy Fluor-405 Conjugated (bs-6098R-BF405)?
KLH conjugated synthetic peptide derived from human PARG1
What are the excitation and emission wavelengths of the AbBy Fluor-405 conjugate (bs-6098R-BF405)?
AbBy Fluor-405 on bs-6098R-BF405: excitation / emission 401nm/421nm.