Lysyl tRNA synthetase Antibody, PE-Cy5 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-6127R-PE-Cy5-100uL |
| Product Name | Lysyl tRNA synthetase Antibody, PE-Cy5 Conjugated |
| Applications |
WB
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Cow, Pig, Horse, Chicken, Rabbit |
| Specifications | |
| Conjugation | PE-Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human KARS2/Lysyl tRNA syntase |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 6850 |
| Subcellular location | Cytoplasm, Nucleus, Secreted, Cell membrane, Extracellular matrix |
| Synonyms | KARS 2; KARS; KARS2; KIAA0070; KRS; Lysine tRNA ligase; Lysine--tRNA ligase; LysRS; Lysyl-tRNA synthetase; SYK_HUMAN. |
| Background | Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. When secreted, acts as a signaling molecule that induces immune response through the activation of monocyte/macrophages. Catalyzes the synthesis of diadenosine oligophosphate (Ap4A), a signaling molecule involved in the activation of MITF transcriptional activity. Interacts with HIV-1 virus GAG protein, facilitating the selective packaging of tRNA(3)(Lys), the primer for reverse transcription initiation. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is Lysyl tRNA synthetase Antibody, PE-Cy5 Conjugated (bs-6127R-PE-Cy5) validated for, and at what dilution?
bs-6127R-PE-Cy5 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-6127R-PE-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Lysyl tRNA synthetase Antibody, PE-Cy5 Conjugated (bs-6127R-PE-Cy5)?
KLH conjugated synthetic peptide derived from human KARS2/Lysyl tRNA syntase
What are the excitation and emission wavelengths of the PE-Cy5 conjugate (bs-6127R-PE-Cy5)?
PE-Cy5 on bs-6127R-PE-Cy5: excitation / emission 488nm/670nm.