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Wnt8A Polyclonal Antibody

Formalin-fixed and paraffin embedded rat brain labeled with Anti-Wnt8A Polyclonal Antibody, Unconjugated (bs-6129R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain labeled with Anti-Wnt8A Polyclonal Antibody, Unconjugated (bs-6129R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: Mouse Heart cell lysates probed with Wnt8A Polyclonal Antibody, Unconjugated (bs-6129R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Heart cell lysates probed with Wnt8A Polyclonal Antibody, Unconjugated (bs-6129R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

  • WB
  • ELISA
  • IHC-P
  • IHC-F
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Mouse
  • Rat

Predicted Reactivity

  • Human
  • Dog
  • Sheep
  • Horse
Overview
Catalog # bs-6129R
Product Name Wnt8A Polyclonal Antibody
Applications WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity Mouse, Rat
Predicted Reactivity Human, Dog, Sheep, Horse
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Wnt8A
Immunogen Range 201-300/351
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 7478
Swiss Prot Q9H1J5
Subcellular location Secreted, Extracellular matrix
Synonyms WNT8D; Protein Wnt-8a; Protein Wnt-8d; WNT8A
Background Ligand for members of the frizzled family of seven transmembrane receptors. May play an important role in the development and differentiation of certain forebrain structures, notably the hippocampus.
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

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