Help

Wnt8A Polyclonal Antibody, FITC Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Mouse
  • Rat

Predicted Reactivity

  • Human
  • Dog
  • Sheep
  • Horse
Overview
Catalog # bs-6129R-FITC
Product Name Wnt8A Polyclonal Antibody, FITC Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Mouse, Rat
Predicted Reactivity Human, Dog, Sheep, Horse
Specifications
Conjugation FITC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Wnt8A
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 7478
Swiss Prot Q9H1J5
Subcellular location Secreted, Extracellular matrix
Synonyms WNT8D; Protein Wnt-8a; Protein Wnt-8d; WNT8A
Background Ligand for members of the frizzled family of seven transmembrane receptors. May play an important role in the development and differentiation of certain forebrain structures, notably the hippocampus.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is Wnt8A Polyclonal Antibody, FITC Conjugated (bs-6129R-FITC) validated for, and at what dilution?

bs-6129R-FITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Wnt8A Polyclonal Antibody, FITC Conjugated (bs-6129R-FITC) react with?

Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog, Sheep, Horse.

How should bs-6129R-FITC be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Wnt8A Polyclonal Antibody, FITC Conjugated (bs-6129R-FITC)?

KLH conjugated synthetic peptide derived from human Wnt8A

What are the excitation and emission wavelengths of the FITC conjugate (bs-6129R-FITC)?

FITC on bs-6129R-FITC: excitation / emission 494nm/518nm.