RBBP4 Polyclonal Antibody, FITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-6130R-FITC |
| Product Name | RBBP4 Polyclonal Antibody, FITC Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human, Chicken, Rabbit, Zebrafish |
| Specifications | |
| Conjugation | FITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human RbAp48 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5928 |
| Subcellular location | Nucleus |
| Synonyms | RBBP4_HUMAN; Histone-binding protein RBBP4; Chromatin assembly factor 1 subunit C; CAF-1 subunit C; Chromatin assembly factor I p48 subunit; CAF-I 48 kDa subunit; CAF-I p48; Nucleosome-remodeling factor subunit RBAP48; Retinoblastoma-binding protein 4; RBBP-4; Retinoblastoma-binding protein p48. |
| Background | RbAp48 (Retinoblastoma-binding protein p48 or Rb-associated protein p48)is a WD repeat protein that is a core histone binding subunit common to several complexes involved in chromatin assembly, chromatin remodeling, and histone deacetylation. These complexes include Chromatin Assembly Factor 1 (CAF1), which is required for chromatin assembly following DNA replication and repair, the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and subsequent transcriptional repression, the nucleosome remodeling and histone deacetylation complex NuRD, the nucleosome remodeling factor (NURF) complex, and the PRC2 complex, which promotes repression of homeotic genes during development. RpAp48 also interacts with the retinoblastoma protein, and with SPEN/MINT and BRCA1. It is also a component of the DREAM complex, which represses cell cycle-dependent genes in quiescent cells. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is RBBP4 Polyclonal Antibody, FITC Conjugated (bs-6130R-FITC) validated for, and at what dilution?
bs-6130R-FITC is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does RBBP4 Polyclonal Antibody, FITC Conjugated (bs-6130R-FITC) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Chicken, Rabbit, Zebrafish.
How should bs-6130R-FITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise RBBP4 Polyclonal Antibody, FITC Conjugated (bs-6130R-FITC)?
KLH conjugated synthetic peptide derived from human RbAp48
What are the excitation and emission wavelengths of the FITC conjugate (bs-6130R-FITC)?
FITC on bs-6130R-FITC: excitation / emission 494nm/518nm.