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WNT2 Polyclonal Antibody

Images provided the Independent Validation Program (badge number 029629)Formalin-fixed and paraffin embedded human brain glioma tissue labeled with Rabbit Anti-WNT2 Polyclonal Antibody (bs-6133R) at 1:250 overnight at 4 \u00b0C followed by conjugation to secondary antibody.
Images provided the Independent Validation Program (badge number 029629)Formalin-fixed and paraffin embedded human brain glioma tissue labeled with Rabbit Anti-WNT2 Polyclonal Antibody (bs-6133R) at 1:250 overnight at 4 \u00b0C followed by conjugation to secondary antibody.
Images provided the Independent Validation Program (badge number 029629)Formalin-fixed and paraffin embedded human brain tissue with Parkinson's morphology labeled with Rabbit Anti-WNT2 Polyclonal Antibody (bs-6133R) at 1:250 overnight at 4 \u00b0C followed by conjugation to secondary antibody.
Images provided the Independent Validation Program (badge number 029629)Formalin-fixed and paraffin embedded human brain tissue with Parkinson's morphology labeled with Rabbit Anti-WNT2 Polyclonal Antibody (bs-6133R) at 1:250 overnight at 4 \u00b0C followed by conjugation to secondary antibody.
Paraformaldehyde-fixed, paraffin embedded Mouse cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with WNT2 Polyclonal Antibody, Unconjugated (bs-6133R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with WNT2 Polyclonal Antibody, Unconjugated (bs-6133R) at 1:200 overnight at 4\u00b0C, DAB staining.

Applications

  • WB
  • ELISA
  • IHC-P
  • IHC-F
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse

Predicted Reactivity

  • Rat
  • Cow
  • Chicken
  • Rabbit
Overview
Catalog # bs-6133R
Product Name WNT2 Polyclonal Antibody
Applications WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity Human, Mouse
Predicted Reactivity Rat, Cow, Chicken, Rabbit
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human WNT2
Immunogen Range 221-320/360
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 7472
Swiss Prot P09544
Subcellular location Secreted
Synonyms IRP; INT1L1; Protein Wnt-2; Int-1-like protein 1; Int-1-related protein; WNT2
Background Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.
Application Dilution
WB 1:500-2000
ELISA 1:5000-10000
IHC-P 1:100-500
IHC-F 1:100-500
IF(IHC-P) 1:100-500
IF(IHC-F) 1:100-500
IF(ICC) 1:100-500

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