Adenovirus 5 E1A Polyclonal Antibody, RBITC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-6136R-RBITC |
| Product Name | Adenovirus 5 E1A Polyclonal Antibody, RBITC Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Virus |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Adenovirus 5 E1A |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Swiss Prot | P03255 |
| Subcellular location | Cytoplasm, Nucleus, Extracellular matrix |
| Synonyms | Adenovirus early E1A protein; Control protein E1A; E1A; Early E1A protein; Human adenovirus type 5; Human adenovirus C; pfam02703; E1A_ADE05. |
| Background | The early region (E1) of the adenovirus genome, responsible for transforming activity, is localized within the left most 11% of the viral genome and consists of two transcriptional units E1A and E1B. E1A is sufficient for partial transformation and immortalization of primary cells. E1A gene products are necessary for normal levels of transcription of the other early regions of the adenovirus genome during productive infection and are able to either activate or repress the transcription of specific cellular genes. E1A forms specific complexes with cellular proteins including p105 causing inhibition of the cell cycle inducing arresting function of p105. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Adenovirus 5 E1A Polyclonal Antibody, RBITC Conjugated (bs-6136R-RBITC) validated for, and at what dilution?
bs-6136R-RBITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Adenovirus 5 E1A Polyclonal Antibody, RBITC Conjugated (bs-6136R-RBITC) react with?
Tested reactivity: Virus.
How should bs-6136R-RBITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Adenovirus 5 E1A Polyclonal Antibody, RBITC Conjugated (bs-6136R-RBITC)?
KLH conjugated synthetic peptide derived from human Adenovirus 5 E1A
What are the excitation and emission wavelengths of the RBITC conjugate (bs-6136R-RBITC)?
RBITC on bs-6136R-RBITC: excitation / emission 540nm/625nm.