KDM5B Polyclonal Antibody, AbBy Fluor-555 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-6139R-BF555-100uL |
| Product Name | KDM5B Polyclonal Antibody, AbBy Fluor-555 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Rat |
| Predicted Reactivity | Mouse, Dog, Cow, Pig, Horse, Chicken, GuineaPig |
| Specifications | |
| Conjugation | AbBy Fluor-555 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human KDM5B/Jarid1B |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 10765 |
| Swiss Prot | Q9UGL1 |
| Subcellular location | Nucleus |
| Synonyms | CT31; PLU1; PUT1; PLU-1; JARID1B; PPP1R98; RBBP2H1A; Lysine-specific demethylase 5B; Cancer/testis antigen 31; Histone demethylase JARID1B; Jumonji/ARID domain-containing protein 1B; Retinoblastoma-binding protein 2 homolog 1; RBP2-H1; KDM5B; RBBP2H1 |
| Background | Histone demethylase that demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9' or H3 'Lys-27'. Demethylates trimethylated, dimethylated and monomethylated H3 'Lys-4'. Acts as a transcriptional corepressor for FOXG1B and PAX9. Favors the proliferation of breast cancer cells by repressing tumor suppressor genes such as BRCA1 and HOXA5. In contrast, may act as a tumor suppressor for melanoma. Represses the CLOCK-ARNTL/BMAL1 heterodimer-mediated transcriptional activation of the core clock component PER2 (By similarity). |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is KDM5B Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-6139R-BF555) validated for, and at what dilution?
bs-6139R-BF555 is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does KDM5B Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-6139R-BF555) react with?
Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Dog, Cow, Pig, Horse, Chicken, GuineaPig.
How should bs-6139R-BF555 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise KDM5B Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-6139R-BF555)?
KLH conjugated synthetic peptide derived from human KDM5B/Jarid1B
What are the excitation and emission wavelengths of the AbBy Fluor-555 conjugate (bs-6139R-BF555)?
AbBy Fluor-555 on bs-6139R-BF555: excitation / emission 553nm/568nm.