RAC1+RAC2 Polyclonal Antibody

Formalin-fixed and paraffin embedded mouse intestine labeled with Anti-RAC1+RAC2 Polyclonal Antibody, Unconjugated (bs-6154R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

$Formalin-fixed and paraffin embedded human lung cancer labeled with Rabbit Anti-RAC1+RAC2 Polyclonal Antibody, Unconjugated (bs-6154R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n$

Formalin-fixed and paraffin embedded human lung cancer labeled with Rabbit Anti-RAC1+RAC2 Polyclonal Antibody, Unconjugated (bs-6154R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n

$Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with RAC1+RAC2 Polyclonal Antibody, Unconjugated (bs-6154R) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with RAC1+RAC2 Polyclonal Antibody, Unconjugated (bs-6154R) at 1:200 overnight at 4\u00b0C, DAB staining.

Applications

ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse

Predicted Reactivity

Rat
Dog
Cow
Sheep
Pig
Horse
Chicken
Rabbit
GuineaPig

Overview
Catalog #	bs-6154R
Product Name	RAC1+RAC2 Polyclonal Antibody
Applications	ELISA IHC-P IHC-F IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity	Human, Mouse
Predicted Reactivity	Rat, Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit, GuineaPig
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human RAC1/RAC2.
Immunogen Range	101-192/192
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	5879, 5880
Subcellular location	Cytoplasm, Cell membrane
Synonyms	MIG5; p21 Rac1; Rac 1; Rac-1; p21 Rac2; PAK1; Ras like protein TC25; Ras related C3 botulinum toxin substrate 1; Ras related C3 botulinum toxin substrate 2; RAC1_HUMAN; RAC2_HUMAN.
Background	The protein encoded by this gene is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009].
Application Dilution
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200