RAC1+RAC2 Polyclonal Antibody, Cy5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-6154R-Cy5 |
| Product Name | RAC1+RAC2 Polyclonal Antibody, Cy5 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat, Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit, GuineaPig |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human RAC1/RAC2. |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5879, 5880 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | MIG5; p21 Rac1; Rac 1; Rac-1; p21 Rac2; PAK1; Ras like protein TC25; Ras related C3 botulinum toxin substrate 1; Ras related C3 botulinum toxin substrate 2; RAC1_HUMAN; RAC2_HUMAN. |
| Background | The protein encoded by this gene is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is RAC1+RAC2 Polyclonal Antibody, Cy5 Conjugated (bs-6154R-Cy5) validated for, and at what dilution?
bs-6154R-Cy5 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does RAC1+RAC2 Polyclonal Antibody, Cy5 Conjugated (bs-6154R-Cy5) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit, GuineaPig.
How should bs-6154R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise RAC1+RAC2 Polyclonal Antibody, Cy5 Conjugated (bs-6154R-Cy5)?
KLH conjugated synthetic peptide derived from human RAC1/RAC2.
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-6154R-Cy5)?
Cy5 on bs-6154R-Cy5: excitation / emission 625,650nm/670nm.