RALA+RALB Polyclonal Antibody, Cy5.5 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-6170R-Cy5.5-100uL |
| Product Name | RALA+RALB Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Cow, Sheep, Pig, Chicken, Rabbit |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human RALA+RALB |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Cytoplasm, Cell membrane, Extracellular matrix |
| Synonyms | Dm RalA; DRal; Drala; Ral; RalA protein; Ras family small GTP binding protein RALA; RAS like family 1; RAS like protein A; RAS LIKE PROTEIN; RAS like protein B; Ras related protein; Ras related protein RalA; Rasl1; Vral simian leukemia viral oncogene homolog A ras related; RALA_HUMAN; RALB_HUMAN. |
| Background | RALA, resorcylic acid lactone alpha, is a low molecular weight GTP binding protein belonging to the RAS family of onco proteins. RALA has a 50% amino acid homology with RAS and an 85% homology with RALB. GTP binding proteins mediate the transmembrane signaling initiated by the occupancy of certain cell surface receptors. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is RALA+RALB Polyclonal Antibody, Cy5.5 Conjugated (BS-6170R-CY5-5) validated for, and at what dilution?
BS-6170R-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should BS-6170R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise RALA+RALB Polyclonal Antibody, Cy5.5 Conjugated (BS-6170R-CY5-5)?
KLH conjugated synthetic peptide derived from human RALA+RALB
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-6170R-CY5-5)?
Cy5.5 on BS-6170R-CY5-5: excitation / emission 675nm/694nm.