Rab3A+Rab3B+Rab3C+Rab3D Antibody

$Paraformaldehyde-fixed, paraffin embedded rat heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Rab3A+Rab3B+Rab3C+Rab3D Antibody Polyclonal Antibody, Unconjugated (bs-6180R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.$

Paraformaldehyde-fixed, paraffin embedded rat heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Rab3A+Rab3B+Rab3C+Rab3D Antibody Polyclonal Antibody, Unconjugated (bs-6180R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

Mouse eye lysates probed with Rab3A+Rab3B+Rab3C+Rab3D Polyclonal Antibody, Unconjugated (bs-6180R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bs-6180R
Product Name	Rab3A+Rab3B+Rab3C+Rab3D Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Rab3A+Rab3B+Rab3C+Rab3D
Immunogen Range	101-150/220
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Subcellular location	Cytoplasm, Cell membrane
Synonyms	RAB3A; RAB3A member RAS oncogene family; RAB3B; RAB3B member RAS oncogene family; RAB3C; RAB3C member RAS oncogene family; RAB3D member RAS oncogene family; RAD3D; Ras related protein Rab 3A; Ras related protein Rab 3B; Ras related protein Rab 3C; Ras related protein Rab 3D; D2 2; Glioblastoma overexpressed; GOV; RAB16.
Background	Rab proteins belong to the Ras small GTPase superfamily. The GTP-binding Rab3 subfamily has four known isoforms: 3A, 3B, 3C and 3D. These proteins are expressed in the highest concentration in the brain and endocrine tissues and are involved in regulated exocytosis of hormones and neurotransmitters. Specifically Rab3's are thought to play a role in presynaptic vesicle trafficking and priming.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200

Applications

Reactivity

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