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SHC3 Polyclonal Antibody

Lane 1: rat heart lysates Lane 2: rat brain lysates probed with Anti SHC3 Polyclonal Antibody, Unconjugated (bs-6199R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 65kD. Observed band size: 65kD
Lane 1: rat heart lysates Lane 2: rat brain lysates probed with Anti SHC3 Polyclonal Antibody, Unconjugated (bs-6199R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 65kD. Observed band size: 65kD
Lane 1: Kindey lysates probed with SHC3 Polyclonal Antibody, Unconjugated (bs-6199R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Kindey lysates probed with SHC3 Polyclonal Antibody, Unconjugated (bs-6199R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

  • WB
  • ELISA
  • IHC-P
  • IHC-F
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Mouse
  • Rat

Predicted Reactivity

  • Human
  • Dog
  • Horse
Overview
Catalog # bs-6199R
Product Name SHC3 Polyclonal Antibody
Applications WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity Mouse, Rat
Predicted Reactivity Human, Dog, Horse
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human SHC3
Immunogen Range 501-594/594
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 53358
Swiss Prot Q92529
Subcellular location Cytoplasm, Cell membrane
Synonyms RAI; NSHC; SHCC; N-Shc; SHC-transforming protein 3; Neuronal Shc; Protein Rai; SHC-transforming protein C; Src homology 2 domain-containing-transforming protein C3; SH2 domain protein C3; SHC3
Background Signaling adapter that couples activated growth factor receptors to signaling pathway in neurons. Involved in the signal transduction pathways of neurotrophin-activated Trk receptors in cortical neurons.
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

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