NCKAP1 Polyclonal Antibody, APC-Cy7 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-6204R-APC-Cy7 |
| Product Name | NCKAP1 Polyclonal Antibody, APC-Cy7 Conjugated |
| Applications |
WB
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat, Dog, Cow, Pig, Horse, Chicken, GuineaPig |
| Specifications | |
| Conjugation | APC-Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human NCKAP1L |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 10787 |
| Swiss Prot | P55160 |
| Subcellular location | Cell membrane |
| Synonyms | NCK associated protein 1; NCK associated protein 1; Membrane associated protein HEM2; Membrane-associated protein HEM-2; NAP 1; NAP1; NAP125; NCK associated protein; Nck-associated protein 1; Nckap1; NCKP1_HUMAN; p125Nap1. |
| Background | NAP125, also known as NCKAP1 (NCK-associated protein 1), p125Nap1 or membrane-associated protein HEM-2, is a 1,128 amino acid single pass membrane protein that exists as two alternatively spliced isoforms and belongs to the HEM-1/HEM-2 family. While widely expressed, NAP125 is found at highest levels in heart, brain and skeletal muscle where it regulates Rac-dependent actin remodeling as part of a lamellipodial complex with WAVE2, Abi-1 and CYFIP1. NAP125 localizes to the cytoplasmic side of lamellipodium membrane and is encoded by a gene that maps to human chromosome 2q32.1 and mouse chromosome 2 C3. NAP125 expression is markedly reduced in Alzheimer disease (AD)-affected brains, suggesting a possible role in the disease. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is NCKAP1 Polyclonal Antibody, APC-Cy7 Conjugated (bs-6204R-APC-Cy7) validated for, and at what dilution?
bs-6204R-APC-Cy7 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does NCKAP1 Polyclonal Antibody, APC-Cy7 Conjugated (bs-6204R-APC-Cy7) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Dog, Cow, Pig, Horse, Chicken, GuineaPig.
How should bs-6204R-APC-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise NCKAP1 Polyclonal Antibody, APC-Cy7 Conjugated (bs-6204R-APC-Cy7)?
KLH conjugated synthetic peptide derived from human NCKAP1L
What are the excitation and emission wavelengths of the APC-Cy7 conjugate (bs-6204R-APC-Cy7)?
APC-Cy7 on bs-6204R-APC-Cy7: excitation / emission 650nm/780nm.