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TMED4 Polyclonal Antibody, FITC Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Mouse
  • Rat

Predicted Reactivity

  • Human
  • Cow
  • Sheep
  • Pig
  • Horse
  • Rabbit
Overview
Catalog # bs-6208R-FITC
Product Name TMED4 Polyclonal Antibody, FITC Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Mouse, Rat
Predicted Reactivity Human, Cow, Sheep, Pig, Horse, Rabbit
Specifications
Conjugation FITC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human TMED4
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 222068
Subcellular location Cytoplasm, Cell membrane
Synonyms HNLF; Transmembrane emp24 domain containing protein 4; TMED4_HUMAN.
Background TMED4 is a putative NF-kB activating protein belonging to the EMP24/GP25L family, and contains 1 GOLD domain. It is also known as Transmembrane emp24 protein transport domain containing 4.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is TMED4 Polyclonal Antibody, FITC Conjugated (bs-6208R-FITC) validated for, and at what dilution?

bs-6208R-FITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does TMED4 Polyclonal Antibody, FITC Conjugated (bs-6208R-FITC) react with?

Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Cow, Sheep, Pig, Horse, Rabbit.

How should bs-6208R-FITC be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise TMED4 Polyclonal Antibody, FITC Conjugated (bs-6208R-FITC)?

KLH conjugated synthetic peptide derived from human TMED4

What are the excitation and emission wavelengths of the FITC conjugate (bs-6208R-FITC)?

FITC on bs-6208R-FITC: excitation / emission 494nm/518nm.