Estrogen Related Receptor gamma Antibody, Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-6213R-Cy5.5-100uL |
| Product Name | Estrogen Related Receptor gamma Antibody, Cy5.5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human, Dog, Cow, Sheep, Pig, Rabbit |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human ESRRG/Estrogen Related Receptor gamma |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 2104 |
| Subcellular location | Nucleus |
| Synonyms | ERR 3; ERR G2; ERR gamma 2; ERR gamma-2; ERR3; ERR3_HUMAN; ERRG 2; ERRG2; ESRRG; Estrogen receptor related protein 3; Estrogen receptor-related protein 3; Estrogen-related receptor gamma; NR3B3; Nuclear receptor subfamily 3 group B member 3. |
| Background | This gene encodes a member of the estrogen receptor-related receptor (ESRR) family, which belongs to the nuclear hormone receptor superfamily. All members of the ESRR family share an almost identical DNA binding domain, which is composed of two C4-type zinc finger motifs. The ESRR members are orphan nuclear receptors; they bind to the estrogen response element and steroidogenic factor 1 response element, and activate genes controlled by both response elements in the absence of any ligands. The ESRR family is closely related to the estrogen receptor (ER) family. They share target genes, co-regulators and promoters, and by targeting the same set of genes, the ESRRs seem to interfere with the ER-mediated estrogen response in various ways. It has been reported that the family member encoded by this gene functions as a transcriptional activator of DNA cytosine-5-methyltransferases 1 (Dnmt1) expression by direct binding to its response elements in the DNMT1 promoters, modulates cell proliferation and estrogen signaling in breast cancer, and negatively regulates bone morphogenetic protein 2-induced osteoblast differentiation and bone formation. Multiple alternatively spliced transcript variants have been identified, which mainly differ at the 5' end and some of which encode protein isoforms differing in the N-terminal region. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Estrogen Related Receptor gamma Antibody, Cy5.5 Conjugated (BS-6213R-CY5-5) validated for, and at what dilution?
BS-6213R-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Estrogen Related Receptor gamma Antibody, Cy5.5 Conjugated (BS-6213R-CY5-5) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog, Cow, Sheep, Pig, Rabbit.
How should BS-6213R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Estrogen Related Receptor gamma Antibody, Cy5.5 Conjugated (BS-6213R-CY5-5)?
KLH conjugated synthetic peptide derived from human ESRRG/Estrogen Related Receptor gamma
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-6213R-CY5-5)?
Cy5.5 on BS-6213R-CY5-5: excitation / emission 675nm/694nm.