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Thyroid hormone receptor alpha Polyclonal Antibody, PE Conjugated

Applications

  • WB
  • FCM
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Sheep
  • Pig
  • Horse
  • Chicken
  • Rabbit
  • GuineaPig
Overview
Catalog # bs-6221R-PE-100uL
Product Name Thyroid hormone receptor alpha Polyclonal Antibody, PE Conjugated
Applications
WB FCM IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Mouse, Rat
Predicted Reactivity Sheep, Pig, Horse, Chicken, Rabbit, GuineaPig
Specifications
Conjugation PE
Host Rabbit
Source KLH conjugated synthetic peptide derived from human THR
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 7067
Swiss Prot P10827
Subcellular location Nucleus
Synonyms Thyroid hormone receptor ; Thyroid hormone receptor alpha; Thyroid hormone receptor beta; Thyroid hormone receptor alpha 1; Thyroid hormone receptor alpha 2; Thyroid hormone receptor beta 1; Thyroid hormone receptor beta 2.
Background Nuclear hormone receptor that can act as a repressor or activator of transcription. High affinity receptor for thyroid hormones, including triiodothyronine and thyroxine.
Application Dilution
WB 1:300-5000
FCM 1:20-100
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Frequently asked questions

Which applications is Thyroid hormone receptor alpha Polyclonal Antibody, PE Conjugated (bs-6221R-PE) validated for, and at what dilution?

bs-6221R-PE is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Thyroid hormone receptor alpha Polyclonal Antibody, PE Conjugated (bs-6221R-PE) react with?

Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Sheep, Pig, Horse, Chicken, Rabbit, GuineaPig.

How should bs-6221R-PE be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Thyroid hormone receptor alpha Polyclonal Antibody, PE Conjugated (bs-6221R-PE)?

KLH conjugated synthetic peptide derived from human THR

What are the excitation and emission wavelengths of the PE conjugate (bs-6221R-PE)?

PE on bs-6221R-PE: excitation / emission 496,564nm/578nm.