phospho-BCAR1 (Tyr410) Polyclonal Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-6224R-100uL |
| Product Name | phospho-BCAR1 (Tyr410) Polyclonal Antibody |
| Applications |
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Specificity | Will recognize BCAR1(Tyr414) in mouse and BCAR1(Tyr508) in rat |
| Reactivity | Rat |
| Predicted Reactivity | Human, Mouse, Horse |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human BCAR1 around the phosphorylation site of Tyr410. |
| Modification Site | Tyr410 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 9564 |
| Swiss Prot | P56945 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | CAS; CAS1; CASS1; CRKAS; P130Cas; Breast cancer anti-estrogen resistance protein 1; CRK-associated substrate; Cas scaffolding protein family member 1; BCAR1 |
| Background | Docking protein which plays a central coordinating role for tyrosine kinase-based signaling related to cell adhesion. Implicated in induction of cell migration. Overexpression confers antiestrogen resistance on breast cancer cells. |
| Application Dilution | |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-BCAR1 (Tyr410) Polyclonal Antibody (bs-6224R) validated for, and at what dilution?
bs-6224R is validated for ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-BCAR1 (Tyr410) Polyclonal Antibody (bs-6224R) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Horse.
How should bs-6224R be stored?
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-BCAR1 (Tyr410) Polyclonal Antibody (bs-6224R)?
KLH conjugated synthetic phosphopeptide derived from human BCAR1 around the phosphorylation site of Tyr410.